By Angela Chen 

DuPont Co. said Monday that it opposes efforts by Trian Fund Management LP to replace four directors, calling the activist investment firm "value destructive."

A representative for Trian wasn't immediately available for comment.

Among the nominees opposed by DuPont is John Myers, the former chief executive of GE Asset Management, part of General Electric Co. In March, DuPont had offered to add Mr. Myers to the board as part of a "constructive resolution."

Monday, DuPont appeared to remove that offer, saying Trian's efforts to replace board members were "not in the best interests of shareholders." The company said Trian's candidates, including co-founder Nelson Peltz, lack experience in the chemicals industry.

Trian has been aiming to shake up the 212-year-old chemicals company and has been pressing for a further split into one company focused on agriculture and nutrition and another focused on industrial materials.

In February, DuPont named two new directors in a board revamp that excluded Trian nominees, ratcheting up the tension in the high-profile battle.

With a market capitalization of $67.5 billion, DuPont ranks among the largest-ever targets of a proxy fight by an activist investor. Trian owns about 2.7% of DuPont's shares.

Before Trian got involved, DuPont had agreed to spin off its performance-chemicals business, which will be named Chemours Co. It expects to return about $4 billion in one-time dividend proceeds from Chemours to shareholders.

Shares of DuPont have been down about 3% this year through Thursday's close.

Write to Angela Chen at angela.chen@dowjones.com

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