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UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 8-K
CURRENT REPORT
Pursuant to Section 13 or 15(d)
of the Securities Exchange Act of 1934
Date of Report (Date of earliest event reported): October 24, 2024
Prelude Therapeutics Incorporated
(Exact Name of Registrant as Specified in its Charter)
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Delaware |
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001-39527 |
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81-1384762 |
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incorporation or organization) |
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(I.R.S. Employer
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175 Innovation Boulevard Wilmington, Delaware |
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19805 |
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Registrant’s telephone number, including area code: (302) 467-1280
Not Applicable
(Former Name or Former Address, if Changed Since Last Report)
Check the appropriate box below if the Form 8-K filing is intended to simultaneously satisfy the filing obligation of the registrant under any of the following provisions:
☐ Written communications pursuant to Rule 425 under the Securities Act (17 CFR 230.425)
☐ Soliciting material pursuant to Rule 14a-12 under the Exchange Act (17 CFR 240.14a-12)
☐ Pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act (17 CFR 240.14d-2(b))
☐ Pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act (17 CFR 240.13e-4(c))
Securities registered pursuant to Section 12(b) of the Act:
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Trading
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Common Stock, $0.0001 par value per share |
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PRLD |
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Nasdaq Global Select Market |
Indicate by check mark whether the registrant is an emerging growth company as defined in Rule 405 of the Securities Act of 1933 (§230.405 of this chapter) or Rule 12b-2 of the Securities Exchange Act of 1934 (§240.12b-2 of this chapter).
Emerging growth company ☒
If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐
Item 8.01 Other events
On October 24, 2024, Prelude Therapeutics Incorporated (the "Company") issued a press release announcing updated interim clinical data from its ongoing Phase 1 trial of PRT3789 and preclinical data from its precision degrader antibody conjugate program deploying a novel SMARCA2/4 dual degrader payload. The press release was issued simultaneously with the previously announced plenary session of the 36th EORTC-NCI-AACR Symposium taking place in Barcelona, Spain. A copy of the press release is attached as Exhibit 99.1 to this report.
In connection with the presentation of the clinical data, the Company has updated its corporate presentation. A copy of the updated corporate presentation is attached as Exhibit 99.2 to this Current Report on Form 8-K and is incorporated herein by reference.
Item 9.01 Financial Statements and Exhibits.
(d) Exhibits
SIGNATURE
Pursuant to the requirements of the Securities Exchange Act of 1934, as amended, the registrant has duly caused this report to be signed on its behalf by the undersigned hereunto duly authorized.
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PRELUDE THERAPEUTICS INCORPORATED |
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Date: October 24, 2024 |
By: |
/s/ Bryant Lim |
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Bryant Lim |
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Chief Legal Officer, Corporate Secretary, and Interim Chief Financial Officer |
Prelude Therapeutics Presents New Data from SMARCA Degrader Portfolio at the 36th EORTC-NCI-AACR Symposium
-Interim data from ongoing trial of PRT3789 showed additional clinical activity at higher doses in patients with non-small cell lung cancer (NSCLC)
-First safety data presented from combination study of PRT3789 and docetaxel demonstrated an acceptable safety profile
-First preclinical proof-of-concept data presented from precision antibody drug conjugate program deploying a novel SMARCA2/4 dual degrader payload
WILMINGTON, Del., Oct. 24, 2024 (GLOBE NEWSWIRE) – Prelude Therapeutics Incorporated (Nasdaq: PRLD) (“Prelude” or the “Company”), a clinical-stage precision oncology company, today announced the presentation of additional data from its ongoing Phase 1 open-label, dose-escalation trial of PRT3789, a first-in-class, highly selective SMARCA2 degrader designed to treat cancer patients with a SMARCA4 mutation. The data were presented at a plenary session of the 36th Annual EORTC-NCI-AACR Symposium in Barcelona, Spain.
The study investigators reported, as of September 23, 2024 (the Cutoff Date), additional follow up data on 65 patients that were enrolled, treated, and safety evaluable. PRT3789 was generally well-tolerated through 8 dosing cohorts. The majority of adverse events reported by investigators have been mild to moderate.
Overall, of the 26 advanced, heavily pre-treated NSCLC or esophageal patients with Class 1 (loss of function) mutations evaluable for efficacy, now with additional follow up, RECIST partial responses (PRs) were confirmed in 4 patients, including 2 of 9 NSCLC patients with confirmed PRs at doses of 283 mg or higher. As anticipated, higher doses are resulting in deeper and more sustained SMARCA2 degradation in PBMCs. Additional patients demonstrated clinical benefit as measured by prolonged stable disease (SD) including one patient on treatment for more than a year.
“We, along with our study investigators, are encouraged by the promising activity shown to date by PRT3789 in this novel first-in-class mechanism for patients who have limited treatment options,” stated Jane Huang, M.D., President and Chief Medical Officer of Prelude. “We look forward to further characterizing and understanding the full potential of PRT3789 through ongoing monotherapy dose escalation and in combination studies with both docetaxel and pembrolizumab.”
PRT3789 Interim Phase 1 Results
PRT3789 is currently being evaluated in an ongoing dose-escalation Phase 1 trial in heavily pre-treated patients with advanced solid tumors harboring any SMARCA4 mutation who have relapsed/refractory disease. Sixty-five patients with advanced cancer were treated once weekly via intravenous infusion at eight dose levels (24 mg, 48 mg, 80 mg, 120 mg, 160 mg, 212 mg, 283 mg, 376 mg), and follow up data reported through to September 23, 2024. The median age of these patients was 62 and the median number of prior treatments was 3 (ranging from 1-10). 34 patients (52.3%) presented with a Class 1 (loss of function) SMARCA4 mutation, while 24 patients (36.9%) presented with a Class 2 (missense, VUS) SMARCA4 mutation and 7 (10.8%) had a loss of SMARCA4 protein.
Initial Safety Data
PRT3789 was generally well-tolerated. Treatment emergent adverse events of any grade observed to date consisted of nausea (26.2%), fatigue (21.5%), anemia (20.0%), decreased appetite (20.0%), abdominal pain (18.5%), and constipation (18.5%). No dose limiting toxicities were observed and no study drug-related serious adverse events were reported.
Pharmacokinetic (PK) and Pharmacodynamic (PD) Data
Preliminary PK data was available from 24 mg to 376 mg dose cohorts. A general trend of increases in exposure (Cmax, AUC) with dose was observed. Mean concentrations were observed above SMARCA2 plasma DC50 (21 nM) for approximately 8 hours at the 376 mg dose. No accumulation was observed with repeat dose administration, consistent with the half-life and once-weekly administration. PD effect (as measured by SMARCA2 protein levels in peripheral blood mononuclear cells) observed was more prolonged than PK half-life. Higher dose levels, above 212 mg, demonstrated a deeper, more consistent, and more prolonged PD effect. SMARCA2 degradation was observed in both peripheral blood mononuclear cells (PBMC) and in available tumor tissue as shown through biopsy.
Analysis of Initial Clinical Activity
Of the 26 advanced NSCLC or esophageal patients with Class 1 (loss of function) mutations who were evaluable for efficacy, RECIST confirmed partial responses (PRs) were observed in 4 patients (2 esophageal, 2 NSCLC), including 2 of 9 NSCLC patients with confirmed PRs at doses of 283 mg or higher. Tumor shrinkage was observed in patients with both Class 1 and Class 2 SMARCA4 mutations. Additional patients on-study demonstrated clinical benefit as measured by prolonged SD, including one advanced NSCLC patient who remains stable and on study having been treated for more than 1 year.
Initial observations of safety from evaluable patients in the PRT3789 plus docetaxel combination dose escalation arm of the trial were also presented. To date, PRT3789 in combination with docetaxel demonstrated an acceptable safety profile, with no dose limiting toxicities or study drug serious adverse events reported.
Additional SMARCA Degrader Presentations
The Company also provided two poster presentations at the conference.
The Selective SMARCA2 Degrader, PRT3789, Counteracts the Protective Cellular Stress Response to Chemotherapy and Enhances the Efficacy of Standard of Care Chemotherapeutic Agents in SMARCA4 Mutant NSCLC Models
The combination of PRT3789 with standard of care NSCLC chemotherapy agents significantly enhances anti-tumor activity in preclinical models of SMARCA4-mutated NSCLC. Downregulation of dominant gene pathways by PRT3789, specifically counters docetaxel-induced upregulation of the E2F and G2/M pathways, resulting in a more comprehensive cell cycle blockade and increased apoptosis in SMARCA4-mutated cells. This synergistic activity was observed with both Class I (loss of function) mutations and Class II (missense) mutations.
Discovery of First-in-Class Precision Antibody Drug Conjugates with a Potent SMARCA 2/4 Dual Degrader Payloads that Safely Achieve Maximal and Tumor Specific Degradation and Efficacy in Mouse Models
PRP0004 is a potent SMARCA2/4 dual degrader that robustly inhibits cancer cell growth and induces cell death. Conjugation of PRP0004 to clinically-validated antibodies yielded novel degrader antibody conjugates (DACs), which demonstrated potent and antigen-selective internalization and SMARCA2 and SMARCA4 degradation in cell lines derived from multiple cancers. Prostate cancer cell lines were among the most sensitive to the PRP0004 degrader payload. PRP0004 downregulated multiple drivers of prostate cancer cell growth and survival and resulted in cell death, rationalizing the use of PSMA-targeting antibodies for initial proof-of-concept studies in preclinical models.
As expected, dosing with PRP0004 on its own was highly efficacious in prostate cancer xenografts but displayed a narrow therapeutic window. However, when delivered as a payload on anti-PSMA antibodies, the anti-PSMA SMARCA2/4 DACs demonstrate robust SMARCA2 and SMARCA4 degradation and antigen-dependent efficacy in xenograft models while being well-tolerated. These data highlight the potential of this first-in-class precision ADC approach utilizing a highly potent SMARCA2/4 dual degrader payload to achieve maximal target degradation in tumors while sparing healthy tissues. This strategy has the potential to significantly expand the reach of Prelude’s novel SMARCA degraders to treating patients without SMARCA4 mutations.
“Preclinical data presented today with our novel approach to develop degrader antibody conjugates by using potent dual degraders of SMARCA2 and 4 as payloads offers first proof-of-concept of effectively and safely targeting an important mechanism to treat cancers beyond those with SMARCA4 mutations” stated Peggy Scherle, Ph.D., Chief Scientific Officer of Prelude.
All of the above noted presentations can be found at Publications - Prelude Therapeutics (preludetx.com).
About PRT3789-01
PRT3789 is a first-in-class, potent and highly selective SMARCA2 degrader, in Phase 1 clinical development in SMARCA4-mutated patients. Enrollment remains on track, and the Company expects to conclude monotherapy dose escalation by year end 2024 and identify the biologically
active dose to advance for future registrational trials. In addition, enrollment of patients into back-fill cohorts enriched for NSCLC and SMARCA4 loss-of-function mutations at higher dose levels is ongoing. The objective is to assess clinical activity in a more homogeneous group of patients with high unmet need to support planned discussions with regulatory agencies. A maximum tolerated dose has not yet been identified. Dose escalation continues, now in the 10th dosing cohort (665 mg IV once weekly).
About Prelude Therapeutics
Prelude Therapeutics is a leading precision oncology company developing innovative medicines in areas of high unmet need for cancer patients. Our pipeline is comprised of several novel drug candidates including first-in-class, highly selective IV and oral SMARCA2 degraders, and a potentially best-in-class CDK9 inhibitor. We are also leveraging our expertise in targeted protein degradation to discover, develop and commercialize next generation degrader antibody conjugates (Precision ADCs) with partners. We are on a mission to extend the promise of precision medicine to every cancer patient in need. For more information, visit preludetx.com.
Cautionary Note Regarding Forward-Looking Statements
This press release contains forward-looking statements within the meaning of the "safe harbor" provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to, anticipated discovery, preclinical and clinical development activities for Prelude’s product candidates, the potential safety, efficacy, benefits and addressable market for Prelude’s product candidates, and clinical trial results for Prelude’s product candidates. All statements other than statements of historical fact are statements that could be deemed forward-looking statements. The words “believes,” “anticipates,” “estimates,” “plans,” “expects,” “intends,” “may,” “could,” “should,” “potential,” “likely,” “projects,” “continue,” “will,” “schedule,” and “would” and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these identifying words. These forward-looking statements are predictions based on the Company’s current expectations and projections about future events and various assumptions. Although Prelude believes that the expectations reflected in such forward-looking statements are reasonable, Prelude cannot guarantee future events, results, actions, levels of activity, performance or achievements, and the timing and results of biotechnology development and potential regulatory approval is inherently uncertain. Forward-looking statements are subject to risks and uncertainties that may cause Prelude's actual activities or results to differ significantly from those expressed in any forward-looking statement, including risks and uncertainties related to Prelude's ability to advance its product candidates, the receipt and timing of potential regulatory designations, approvals and commercialization of product candidates, clinical trial sites and our ability to enroll eligible patients, supply chain and manufacturing facilities, Prelude’s ability to maintain and recognize the benefits of certain designations received by product candidates, the timing and results of preclinical and clinical trials, Prelude's ability to fund development activities and achieve development goals, Prelude's ability to protect intellectual property, and other risks and uncertainties described under the heading "Risk Factors" in Prelude’s Annual Report on Form 10-K for the year ended December 31, 2023, its Quarterly Reports on Form 10-Q and other documents that Prelude files from time to time with the Securities and Exchange Commission. These forward-looking statements speak only as of the date of this press release, and Prelude
undertakes no obligation to revise or update any forward-looking statements to reflect events or circumstances after the date hereof, except as may be required by law.
Investor Contact:
Robert A. Doody Jr.
Senior Vice President, Investor Relations
484.639.7235
rdoody@preludetx.com
Corporate Presentation October 2024 Exhibit 99.2
Forward Looking Statements This presentation contains “forward-looking” statements within the meaning of the “safe harbor” provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to, anticipated discovery, preclinical and clinical development activities for Prelude’s product candidates, the potential safety, efficacy, benefits and addressable market for Prelude’s product candidates, the expected timeline for clinical trial results for Prelude’s product candidates including its SMARCA2 degrader molecules. Any statements contained herein or provided orally that are not statements of historical fact may be deemed to be forward-looking statements. In some cases, you can identify forward-looking statements by such terminology as ‘‘believe,’’ ‘‘may,’’ ‘‘will,’’ ‘‘potentially,’’ ‘‘estimate,’’ ‘‘continue,’’ ‘‘anticipate,’’ ‘‘intend,’’ ‘‘could,’’ ‘‘would,’’ ‘‘project,’’ ‘‘plan,’’ ‘‘expect’’ and similar expressions that convey uncertainty of future events or outcomes, although not all forward-looking statements contain these words. Statements, including forward-looking statements, speak only to the date they are provided (unless an earlier date is indicated). This presentation shall not constitute an offer to sell or the solicitation of an offer to buy, nor shall there be any sale of these securities in any state or other jurisdiction in which such offer, solicitation or sale would be unlawful prior to registration or qualification under the securities laws of any such state or other jurisdiction. These forward-looking statements are based on the beliefs of our management as well as assumptions made by and information currently available to us. Although we believe the expectations reflected in such forward-looking statements are reasonable, we can give no assurance that such expectations will prove to be correct. If such assumptions do not fully materialize or prove incorrect, the events or circumstances referred to in the forward-looking statements may not occur. We undertake no obligation to update publicly any forward-looking statements for any reason after the date of this presentation to conform these statements to actual results or to changes in our expectations, except as required by law. Accordingly, readers are cautioned not to place undue reliance on these forward-looking statements. Additional risks and uncertainties that could affect our business are included under the caption “Risk Factors” in our filings with the Securities and Exchange Commission, including our Annual Report on Form 10-K for the year ended December 31, 2023.
We are on a mission to extend the promise of precision medicine to every cancer patient in need Select the best modality to precisely target oncogenic mechanisms Strive for first- or best-in-class and anchor to patient unmet need Draw on decades of experience and proven leadership to drive innovation
Kris Vaddi, PhD Chief Executive Officer Jane Huang M.D. President and Chief Medical Officer Andrew Combs, PhD Chief Chemistry Officer Sean Brusky, MBA Chief Business Officer Peggy Scherle, PhD Chief Scientific Officer Bryant Lim, J.D. Chief Legal Officer, Corporate Secretary and Interim CFO Experienced Leadership Team With Proven Track Records in Precision Oncology
Prelude’s Evolution Strategic Priorities Continue to build SMARCA leadership Generate proof-of-concept data Prepare for global registrational trials Advance SMARCA “Pipeline in a Program” Explore collaborations to accelerate trials and global capabilities ~1 new IND every 12-18 months Successfully advance programs into early clinical development Assembled team to create a highly productive discovery engine Delivered initial wave of first- or potentially best-in-class clinical development candidates: PRMT5i, MCL1i, CDK9i, CDK4/6i, SMARCA2 degraders Continue to grow R&D team while adding key capabilities for future growth Expand global clinical development footprint and capabilities Advance lead clinical development candidates to registrational trials Advancing clinical programs including IV SMARCA2 degrader (PRT3789), oral SMARCA2 degrader (PRT7732) and CDK9 inhibitor (PRT2527) towards PoC Developing SMARCA as ‘Pipeline in Program’ with IV, Oral and ‘Precision ADC’ Approaches 2016 – 2022 2022 – 2025 2025+ Establish Leading Precision Oncology Discovery Engine Expand Development Capabilities, Strategic Focus on SMARCA Advance to Registrational Trials, Demonstrate Value
Prelude’s Precision Medicine Pipeline & Discovery Engine PROGRAM POTENTIAL INDICATIONS DISCOVERY PHASE 1 PHASE 2/3 Lead SMARCA2 Degrader (IV) SMARCA4-mutated NSCLC & other cancers Oral SMARCA2 Degrader SMARCA4-mutated NSCLC & other cancers SMARCA2/4 Precision ADCs* Dose Confirmation by YE2024; Phase 2 Pembrolizumab Combo Trial Start in Q4 2024 UPCOMING MILESTONES First Pre-clinical PoC Data Presented at ENA; Additional Data in 2025 Phase I Trial Initiated PRT3789 Next-Gen CDK9 Selective Inhibitor Myeloid and Lymphoid malignancies PRT2527 Interim Phase 1 Data Anticipated in Q4 2024 Discovery Engine Deliver a First- or Best-in-Class New Program Every 12-18 Months Hard-to-treat cancers, “undruggable” targets, high unmet need Precision ADCs* Broad range of cancers (heme & solid tumors) Broad range of cancers (heme & solid tumors) * Precision ADCs are the focus of our strategic collaboration with AbCellera PRT7732 Advance Additional Novel Payload-Antibody Pairings
Developing an Industry Leading Portfolio of SMARCA-Targeted Precision Medicines SMARCA - DACs + Lead SMARCA2 Degrader (PRT3789) Oral SMARCA2 Degrader (PRT7732) Precision ADCs with SMARCA2/4 Degrader Payload
Patients with SMARCA4 mutations are not typically eligible for other targeted therapies Currently treated with standard of care chemotherapy or chemo-immunotherapy Targeting SMARCA4-mutated Cancer By Selectively Degrading SMARCA2 Pancreatic: 3% NSCLC: 10% Esophageal: 8% Gastric: 8% Endometrial: 13% SCLC: 8% Urinary: 9% Colorectal: 6% Dagogo-Jack et al. Journal of Thoracic Oncology. 2020 Foundation Medicine Dataset SMARCA4 (BRG1) mutations occur in approximately 5% of all cancers Mutations in the chromatin remodeling complex drive cancer growth and resistance Cancer cells with deleterious SMARCA4 mutations become highly dependent on SMARCA2 for survival Selectively degrading SMARCA2 induces "synthetic lethality" in SMARCA4-deficient cancers
Outcomes for Patients with SMARCA4-mutated NSCLC are Poor with Current Standard of Care Alessi JV, et al. Clinicopathologic and genomic factors impacting efficacy of first-line chemoimmunotherapy in advanced non-small cell lung cancer (NSCLC). J Thorac Oncol. 2023 Feb 10:S1556-0864(23)00121-1. PMID: 36775193. Median progression free survival for first-line SMARCA4-mutated NSCLC treated with chemoimmunotherapy is 2.7 months and response rates approximately 22% There is even greater unmet need in second-line and beyond Patients treated with first-line chemoimmunotherapy
PRT3789: A Highly Potent SMARCA2 Degrader with >1000-fold Selectivity Over SMARCA4 Hulse et al. Cancer Res. (2022); 82 (12_Suppl) :3263. AACR 2022 Poster (http://www.preludetx.com/science/publications) Highly selective for SMARCA2 vs SMARCA4 (>1000 fold) and selective across the proteome Preclinical Assay PRT3789 SMARCA2 Degradation (nM) 0.73 Selectivity: Cell Proliferation (SMARCA4 / SMARCA2) >1000 fold Sub-nanomolar SMARCA2 degradation potency in cell lines Anti-tumor activity, including regressions, in SMARCA4 mutant models in vivo PRT3789 Tumor Regression PRT3789 Vehicle Days of Dosing
PRT7732: First-in-Class, Highly Selective Oral SMARCA2 Degrader – Phase I Trial Initiated Sub-nanomolar SMARCA2 degradation potency in cell lines Very high selectivity for SMARCA2 over SMARCA4 Good oral bioavailability observed across species supports projected once-daily human dose Assay PRT7732 SMARCA2 Degradation (nM) 0.98 Selectivity: Degradation (SMARCA4 / SMARCA2) >3000 fold Selectivity: Cell Proliferation (SMARCA4 / SMARCA2) >1000 fold ClinicalTrials.gov Identifier: NCT06560645 Patient Population Dose Finding/Expansion PRT7732 Monotherapy SMARCA4-mutated Solid Tumors Backfill Cohorts to Confirm Biologically Active Dose and Inform Registration Path Goal: Establish Initial Proof-of-Concept and Confirm Biologically Active Dose as Monotherapy
Interim Update from PRT3789-01 Presented at Plenary Session of the 2024 ENA Symposium Additional clinical activity observed in NSCLC patients with Class I mutations treated with PRT3789 monotherapy at doses ≥ 283 mg First look at safety and PK data from PRT3789 + docetaxel in combination demonstrate acceptable safety profile, with no dose limiting toxicities to date 2024 Triple Meeting Update ClinicalTrials.gov Identifier: NCT05639751 Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Study Schema and Enrollment Study is enrolling patients with evaluable disease, any solid tumors, and any type of SMARCA4 mutation Dose escalation is on-going, now at cohort 10 (665 mg) Data presented includes additional follow-up on 65 patients treated in escalating doses from 24 to 376 mg, including backfills enriched for NSCLC with Class 1 (LOF) mutations 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
65 patients with additional follow-up included in the analysis were treated and safety evaluable at time of data cutoff The primary tumor type, as characterized by investigators, was NSCLC (n = 30) along with other solid tumors 34 patients had Class 1 (loss of function) mutations and an additional 7 patients had loss of SMARCA4 protein by IHC PRT3789-01: Demographics and Disease Characteristics, PRT3789 Monotherapy VUS, variant of uncertain significance; IHC, immunohistochemistry. 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024 Characteristics Patients (N=65) Age (years) Median 62 Sex, n (%) Male 36 (55.5) Female 29 (44.6) Prior lines of systemic anti-cancer therapy, n Median (min, max) 3 (1, 10) Tumor type, n (%) Non-small cell lung cancer 30 (46.2) Pancreatic cancer 6 (9.2) Breast cancer 4 (6.2) Gastric cancer/small intestine cancer 3 (4.6) Thoracic undifferentiated 3 (4.6) Cholangiocarcinoma 2 (3.1) Colorectal cancer 2 (3.1) Esophageal cancer 2 (3.1) Other 13 (20.0) Type of SMARCA4 mutation, n (%) Class 1 (loss of function) 34 (52.3) Class 2 (missense, VUS) 24 (36.9) Loss of SMARCA4 protein (BRG1) by IHC 7 (10.8) Note: For the ENA analysis, 4 patients previously listed as NSCLC were reclassified as “thoracic undifferentiated” or “other”. Patients with at least 7 weeks of follow-up are included.
PRT3789-01: Summary of Adverse Events PRT3789 was generally well tolerated at doses studied with no treatment related SAEs or dose-limiting toxicities reported Of all Treatment Emergent Adverse Events (TEAEs) of any grade, nausea, fatigue, anemia and decreased appetite had the highest incidence 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Phase 1 Interim PK Findings Preliminary PK data are available from 24 mg to 376 mg General trend of increases in exposure (Cmax, AUC) with higher doses was observed At the 376 mg dose level, mean concentrations were above SMARCA2 plasma DC50 (21 nM) for approximately 8 hours As expected with a potent degrader, the observed pharmacodynamic effect was more prolonged than pharmacokinetic half-life 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
At dose levels up to 160 mg, degradation of SMARCA2 was observed in PBMCs at early time points, but recovered or was above baseline by the end of the dosing interval (7 days) At dose levels 212 – 376 mg, greater consistency, dose dependency, and sustained degradation of SMARCA2 were observed throughout the treatment cycle Note: LLQ (Lower Limit of Quantification) values were used for any value BLQ (Below Limit of Quantification). Source: Data on file. PBMC, peripheral blood mononuclear cells. PRT3789-01: SMARCA2 Protein Levels in PBMCs At Dose Levels 212 – 376 mg At Dose Levels 24 – 160 mg
In preclinical models, correlation was observed between PBMC and tumor SMARCA2 degradation levels at efficacious doses Increasing doses resulted in increased reduction in SMARCA2 PD AUC in tumors and were associated with higher efficacy PD Correlates with Efficacy in Preclinical Models In vivo Efficacy Tumor levels from mouse xenograft model and PBMC levels from normal rat after single doses that provide equivalent and efficacious exposure AUC, area under curve; PBMC, peripheral blood mononuclear cells. Source: Wang et. al. ENA 2023 Poster (http://www.preludetx.com/science/publications); Data on file SMARCA2 Levels over Time After a Single IV Dose of PRT3789 PD AUC/Efficacy Correlation 50 mg/kg = 243 human dose equivalent 75 mg/kg = 365 mg human dose equivalent 100 mg/kg = 487 mg human dose equivalent
PRT3789-01: Phase 1 Interim Clinical Activity Of the 29 patients with NSCLC or esophageal cancer evaluable for efficacy at time of data cutoff, 8 experienced tumor shrinkage RECIST confirmed partial responses (PRs) were observed in 4 patients (2 esophageal, 2 NSCLC) The two NSCLC responders had Class I mutations and were treated at the 283 mg dose level Stable disease was observed in patients with both Class 1 and Class 2 SMARCA4 mutations 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Phase 1 Interim Clinical Activity As reported by Alessi et al., the median PFS for first-line SMARCA4-mutated NSCLC treated with chemoimmunotherapy was 2.7 months 1 Several patients had prolonged stable disease (SD) including a NSCLC patient who remains on treatment for more than a year 1 Alessi JV, et al. Clinicopathologic and genomic factors impacting efficacy of first-line chemoimmunotherapy in advanced non-small cell lung cancer (NSCLC). J Thorac Oncol. 2023 Feb 10:S1556-0864(23)00121-1. PMID: 36775193. 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Response Rate By Dose Level As reported by Alessi et al., the objective response rate (ORR) for first-line SMARCA4-mutated NSCLC treated with chemoimmunotherapy was 21.9% 1 At doses ≥ 283 mg, as monotherapy, an interim ORR of 22.2% was observed in NSCLC patients with Class I SMARCA4-mutations 2024 Triple Meeting Update 1 Alessi JV, et al. Clinicopathologic and genomic factors impacting efficacy of first-line chemoimmunotherapy in advanced non-small cell lung cancer (NSCLC). J Thorac Oncol. 2023 Feb 10:S1556-0864(23)00121-1. PMID: 36775193. Note: Table includes all efficacy evaluable patients with NSCLC or esophageal cancer with Class 1 mutations, with or without a post-baseline scan Esophageal NSCLC Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Docetaxel Combination Study Schema At time of data cutoff, 11 patients treated at 120 mg (n=6) and 283 mg (n=5) were evaluable for preliminary safety and PK assessment Enrollment continues with no dose limiting toxicities observed to date and is now enrolling at 500 mg Backfill cohorts enriched for NSCLC and Class I LOF mutations are also enrolling 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Preliminary Safety and Adverse Event Summary in Combination with Docetaxel In combination with docetaxel, PRT3789 was generally well tolerated at doses studied with no treatment related SAEs or dose-limiting toxicities reported Most frequent treatment emergent AEs of any grade included fatigue, neutropenia and diarrhea 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
PRT3789-01: Preliminary PK Assessment in Combination with Docetaxel At time of data cutoff, preliminary PK data was available from 6 patients in the 120 mg combination cohort PRT3789 PK in combination with docetaxel appears to be consistent with monotherapy at 120 mg Early signs of anti-tumor activity reported by investigators Additional data to be presented at a major medical meeting in 2025 2024 Triple Meeting Update Yap, T. et al., ENA (EORTC, NCI, AACR) 36th Symposium, October 24th 2024 Data cutoff: 23 September 2024
KEYTRUDA® is a registered trademark of Merck Sharp & Dohme LLC, a subsidiary of Merck & Co., Inc., Rahway, NJ, USA. LOF = “Loss-of-function”; QW, once weekly; YE, year end; NSCLC, non-small cell lung cancer; 2L+, second-line and beyond. What’s Next for PRT3789? ‘3789 Monotherapy Dose Confirmation ‘3789 + KEYTRUDA® ‘3789 Program Priorities: Confirm biologically active dose as monotherapy Further characterize activity in Class 1 (LOF) vs. Class 2 patients at biologically active doses Share initial clinical activity data on combination with docetaxel in 2025 Currently enrolling patients in dose escalation cohort 10 (665 mg QW) Backfill cohorts continue to enroll Enriching for NSCLC and esophageal cancer w/ Class I LOF mutations Expecting dose confirmation by YE24 Additional information on clinical activity at higher doses to be presented in 2025 ‘3789 + Docetaxel Phase 2 pembrolizumab combination trial is initiated Subject of collaboration agreement with Merck Goal is to assess safety and clinical activity in combination Docetaxel combination cohorts continue to enroll Goal is to assess safety and clinical activity in combination Docetaxel is the chemotherapy most often used in 2L+ NSCLC Seeking to improve upon poor outcomes observed with current standard of care
Prelude’s SMARCA2 Degrader Portfolio Addresses a Significant Unmet Need ~195,000 pts/year Up to 19,500 SMARCA4-mutated STAGE IV, First Line ~130,000 pts/year Up to 13,000 SMARCA4-mutated STAGE IV, 2L+ (R/R, Previously Treated) + US & EU5 only (2030 proj.): 1 GlobalData (SEER), Earlier Stage (I-III) includes incidence only, Stage IV includes drug-treated prevalence only, with progression from earlier stages; all three factor-out patients treated with targeted therapies for driver mutations; 2 Datamonitor 2023 Lung Cancer Report; 3 Cerner CancerMpact Tumor Type Reports 2024 4 Schoenfeld et al. Clin Cancer Res. (2020); 26(21):5701-5708. 5 Dagogo-Jack et al. J Thorac Oncol. (2020); 15(5):766-776.; Analysis on File. ~235,000 pts/year Up to 23,500 SMARCA4-mutated EARLIER STAGE (I-III) (Adjuvant / Neo-Adj.) + / Potential Addressable Patient Populations US and EU5 1-5 + / Other SMARCA4-mutated Cancers TBD based on selected tumors 3,4 NSCLC SMARCA4 mutations occur in ~5% of all cancers 5 Up to 10% SMARCA4-mutated 5 Stage IV, Advanced Disease + Up to ~8% SMARCA4-mutated 5 EC/GEJ/GC Stage IV, 2L+ ~35,000 pts/year Up to 7,500 SMARCA4-mutated Stage IV, 1L ~62,000 pts/year Relative diagnosed prevalence in Asia: 8-10X US / EU5 1 Pan-Tumor
Expanding Our Portfolio of SMARCA-Targeted Precision Medicines Precision ADCs with SMARCA2/4 Degrader Payload Cancers highly sensitive to SMARCA dysregulation Independent of SMARCA4 mutation status Initial focus of Prelude/AbCellera collaboration SMARCA - DACs + Lead SMARCA2 Degrader (PRT3789) Oral SMARCA2 Degrader (PRT7732)
Together, Prelude and AbCellera Are Creating Novel, First-in-Class Precision ADCs High Unmet Need & Opportunity Potential for Optimized Payload Potential for Optimized Antibody Data presented describe the first preclinical proof-of-concept of a novel, highly potent SMARCA2/4 dual degrader as a “Precision Payload” conjugated to multiple antibodies Prelude’s SMARCA2/4 dual degraders have shown picomolar potency with potential for increased efficacy, selectivity and improved therapeutic index Precision ADCs have potential to expand the reach of SMARCA degrader technology to cancers without SMARCA4 mutations * Antibody target and tumor type(s) for initial candidates remain undisclosed at this time 2024 Triple Meeting Update
Identification of Selective SMARCA2/4 Dual Degraders with Potent Anti-Cancer Activity (A) SMARCA2/4 degradation potency of 3 payloads in a HeLa HiBiT cell-based assay. (B) Global proteomics analysis following treatment of LNCaP human prostate cancer cells with 25 nM PRP0004 for 1h. (C) GI50 of a panel of cancer cell lines treated with PRP0004, assessed by CellTiter-Glo® assay. 2024 Triple Meeting Update Carter J.,. et al., 2024 EORTC, NCI, AACR Symposium Poster (http://www.preludetx.com/science/publications) Prelude has optimized several highly potent and selective SMARCA2/4 dual degraders for use as novel payloads in degrader antibody conjugates (DACs) PRP0004 is a potent SMARCA2/4 dual degrader that is highly selective for SMARCA2 and SMARCA4 across the proteome PRP0004 robustly inhibits cancer growth and induces cell death across a range of cancer cell lines tested
Conjugation of Clinically-Validated Antibodies to SMARCA2/4 Degrader Payloads Drives Antigen-Dependent Internalization and Target Engagement Conjugation of PRP0004 to clinically-validated antibodies including PSMA, CEACAM5, C-MET, TROP2, and CD33 These DACs demonstrated potent and antigen-selective internalization and target engagement across multiple cancer types Prostate cancer was amongst the most sensitive cell lines to SMARCA2/4 degradation rationalizing the use of PSMA-targeting antibodies for further proof-of-concept studies 2024 Triple Meeting Update Carter J.,. et al., 2024 EORTC, NCI, AACR Symposium Poster (http://www.preludetx.com/science/publications)
Anti-PSMA SMARCA2/4 DAC Demonstrated Tumor Regression and Significantly Better Efficacy Compared to a Traditional PSMA-Targeted Cytotoxic ADC Anti-PSMA SMARCA2/4 DACs demonstrated robust target engagement and antigen-dependent efficacy in xenograft models while being well-tolerated These data highlight the potential of utilizing a SMARCA2/4 degrader payload to achieve maximal target degradation in tumors while sparing healthy tissues Precision ADCs have the potential to expand the therapeutic reach of SMARCA2/4 degraders to patients without SMARCA4 mutations 2024 Triple Meeting Update (A) SMARCA2/4 protein expression was analyzed in DAC PRA0002 and payload PRP0004-treated LNCaP tumors at the indicated time points following a single dose. Graphs are quantitation of western blots. (B) Weekly i.v. administration of PRA0002 was well-tolerated and demonstrated significant tumor growth inhibition (89%) of PSMA+ LNCaP tumors. (C) Weekly i.v. administration of PRA0002 did not induce significant tumor growth inhibition in PSMA- PC3 tumors, in comparison to PRP0004 which was efficacious, but caused mouse body weight loss and death (D) Weekly i.v. administration of PRA0009 demonstrated tumor regression and significantly better efficacy compared to a PSMA cytotoxic ADC (Rosopatamab-MC-MMAF, DAR2) in LNCaP tumors. Carter J.,. et al., 2024 EORTC, NCI, AACR Symposium Poster (http://www.preludetx.com/science/publications)
Highly Selective CDK9 Inhibitor PRT2527
Initial safety and tolerability data for monotherapy dose escalation cohorts in hematologic malignancies Initial assessment of clinical activity in B-cell malignancies as monotherapy Initial clinical data with zanubrutinib from combination cohort *R/R disease following: At least 1 prior systemic therapy for aggressive BCL subtypes, MCL and Richter’s syndrome; At least 2 prior therapies including a BTK inhibitor and venetoclax for CLL. ClinicalTrials.gov Identifier: NCT05159518 Phase 1 Trial of PRT2527 in Hematologic Malignancies is Underway Patient Population* Dose Finding/Expansion Monotherapy PRT2527 Select R/R B-cell, T-cell and myeloid malignancies: Aggressive B-cell lymphoma subtypes, MCL, CLL/SLL including Richter syndrome, AML, and T-cell lymphoma subtypes PRT2527 Combinations What to Expect in Q4 2024 B-cell, T-cell, myeloid malignancies Lymphoid malignancies (combo with zanubrutinib) Myeloid malignancies (combo with venetoclax) Goal: Establish Initial PoC and Identify Mono and/or Combination Recommended Doses for Expansion
Continued Execution Across Strategic Priorities PRT2527 CDK9 Initiate zanubrutinib combination study Initiate myeloid cohort in the existing phase 1 study Complete monotherapy dose escalation in B-cell malignancies Report interim phase 1 clinical results in 2024 PROGRAM Report interim Phase 1 clinical results in 2H 2024 (ESMO & ENA) Initiate Phase 2 trial in combination with pembrolizumab Complete monotherapy escalation and fully enroll backfill cohorts PRT3789 Lead IV SMARCA2 Degrader Selective CDK9 Inhibitor PRT2527 Investigational New Drug (IND) authorization from FDA Initiate Phase 1 in patients with SMARCA4 mutations Report interim Phase 1 clinical results PRT7732 Oral SMARCA2 Degrader Discovery Engine Precision ADCs & Other Advance next first-in-class, novel small molecule discovery candidate Advance first SMARCA2/4 Precision ADC in partnership with AbCellera Advance second Precision ADC program in partnership with AbCellera EXPECTED DELIVERABLE MILESTONE Complete Complete YE 2024 Complete Complete 2025 Complete Complete 2H 2024 Q4 2024 2024 2025 2025 Cash, Cash Equivalents of $179.8 Million as of 6/30/2024
Thank You Contact Us: Robert Doody SVP, Investor Relations rdoody@preludetx.com
Highly Selective SMARCA2 Degrader Program Discovery Effort & Oral Degrader Program (PRT7732) Preclinical Rationale for Combinations (2024 EORTC-NCI-AACR Symposium Update) Current Treatment Paradigm & Testing Landscape Precision ADCs First Preclinical Proof-of-Concept Data Presented at 2024 EORTC-NCI-AACR Symposium Overview of Prelude’s Precision ADC Program and Next Steps CDK9 Program for Hematologic Malignancies (PRT2527) Background, Unmet Need and Scientific Rationale Early Clinical Safety and PK/PD Data from Phase I Study in Solid Tumors Interim Phase I Update Planned for Major Medical Meeting in Q4 2024 APPENDIX BOLD = New data included in Appendix with this update
When it Comes to Targeting SMARCA2, Degraders Offer Distinct Advantages Inhibitors Degraders Potency High Selectivity Extended PD Oral Bioavailability X X Early attempts at achieving both potency and selectivity with inhibitor approaches had challenges Inhibitors do not degrade the target and need to be dosed at levels that retain IC90 coverage continuously Degraders demonstrate sustained PD effect as it takes 48-72h for SMARCA2 to resynthesize
Selectively Targeting SMARCA2 Has Been a Significant Challenge for Industry Selective SMARCA2 Inhibition is an Unmet Medicinal Chemistry Challenge Bromodomain Binders Non-selective and inactive in SMARCA4 mutated cancer cells1 ATPase Inhibitors Inhibitors show low selectivity for SMARCA2 in cell proliferation assays (<10 fold2 and ~33 fold3) ATPase domain Prelude’s Targeted Protein Degradation (TPD) Approach SMARCA2 Selective Degradation is possible through differences in ternary complexes and subsequent ubiquitination of unique lysine residues 1 Vangamudi et al, Cancer Res. 2015 (Pfizer); Taylor et al, J. Med. Chem 2022 (Genentech) 2 Papillon et al, J. Med. Chem 2018 (Novartis) 3 AACR 2024 (Foghorn/Lilly)
Prelude Scientists Solved the SMARCA2 Selectivity Enigma PRT3789 (IV or SC formulation) PRT7732 (Oral Candidate) Parallel VHL- and CRBN-based SMARCA2 Degrader Programs IV or SC Candidate - VHL-TPDs provided an expedited path to potential clinical development with QW dosing Oral Candidate - CRBN-TPDs provided oral candidates, but required extensive lead optimization with balancing of potency, selectivity and oral PK properties Our lead IV and oral clinical candidates both have sub-nanomolar degradation potencies and very high selectivity (>1000 fold) for SMARCA2 over SMARCA4
Our SMARCA2 Oral Degrader Program Progressed Rapidly and Systematically Confidential Solving for potency, selectivity and oral bioavailability was a challenge PRT7732: Lead Oral Candidate with >3000-fold Selectivity A and B: Two additional structurally distinct oral back-up candidates 0.1 1.0 10 100 1000 SMARCA 2 HeLa HiBit DC50 (nM) PRT7732 SMARCA2 HiBit DC50 & SMARCA4 Selectivity A B Note: Inactive & weakly potent compounds removed for clarity Program Progression
Tertiary Complex of SMARCA2/ PRT7732/CRBN-DDB1 E3 Ligase PRT7732 binds to the SMARCA2 bromodomain and CRBN/DDB1 E3 ligase complex PRT7732 has been shown to catalyze the polyubiquitination of unique lysine residues expressed only in SMARCA2 and not SMARCA4 Unique conformational bias promotes selective ubiquitination and degradation of SMARCA2 PRT7732: Our Lead Oral SMARCA2 Degrader Shvartsbart, K. Ito et al., AACR Poster, April 2024. (http://www.preludetx.com/science/publications)
PRT7732 is Highly Potent and Orally Bioavailable With Near-Absolute Selectivity for SMARCA2 Near-absolute cellular selectivity for SMARCA2 vs SMARCA4 (>3000 fold) in HiBit cell lines and >1000-fold in cell proliferation assays Sub-nanomolar SMARCA2 degradation potency * Based on highest concentration tested Good oral bioavailability observed across species supporting once-daily projected human dose Preclinical Assay PRT7732 SMARCA2 Degradation (nM) 0.98 Selectivity: Degradation (SMARCA4 / SMARCA2) >3000 fold Selectivity: Cell Proliferation (SMARCA4 / SMARCA2) >1000 fold* Shvartsbart, K. Ito et al., AACR Poster, April 2024. (http://www.preludetx.com/science/publications)
PRT7732 Has Significant Anti-Tumor Activity in SMARCA4-Deficient Cancer Xenograft Models Daily oral administration of PRT7732 demonstrates anti-tumor activity in SMARCA4-deficient but not SMARCA4 wild type tumors PRT7732 rapidly decreases SMARCA2 protein levels in tumor xenograft models at low doses Preclinical data supported advancing PRT7732 to Phase I with once-daily dosing Shvartsbart, K. Ito et al., AACR Poster, April 2024. (http://www.preludetx.com/science/publications)
PRT3789 Demonstrates Potential for Synergy with Chemotherapy and Apoptosis-Inducing Agents AACR 2022, 2023 Posters (http://www.preludetx.com/science/publications) BUB1B, CCNA2/B2, CDC25C, CDK1/2, CHEK1/2 FEN1, LIG1, MCM, PCNA, POLA1/2, POLD1/2/3, POLE/2/3 Docetaxel Gemcitabine Several oncogenic gene sets regulated by PRT3789 Supports combination strategies with both cytotoxic and apoptosis-inducing agents (e.g., RAS) In vivo CDX models show strong tumor regression in combination with gemcitabine or docetaxel
PRT3789 + Taxanes Induce Durable Regressions in SMARCA4-mutated NSCLC CDX & PDX Models Hulse M., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications) PRT3789 enhances chemotherapy efficacy as shown in NSCLC models with SMARCA4 mutations, including both cell line-derived and patient-derived xenografts PRT3789 significantly improved the efficacy of standard-of-care taxane chemotherapy agents (docetaxel or nab-paclitaxel) 2024 Triple Meeting Update
Preclinical PK/PD Data Shows No Adverse Drug-Drug Interaction Between PRT3789 and Taxanes PRT3789 treatment results in complete degradation of SMARCA2 protein as both monotherapy and in combination with docetaxel Taxanes do not interfere with the SMARCA2 degradation induced by PRT3789 in vivo PK analysis of mouse plasma reveals no adverse drug-drug interactions (DDIs) between PRT3789 and docetaxel Exposure of docetaxel is not affected by combination with PRT3789 2024 Triple Meeting Update Hulse M., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
PRT3789 and Docetaxel Regulate Distinct Pathways Involved in Tumor Cell Growth and Apoptosis 2024 Triple Meeting Update Hulse M., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
PRT3789 Counteracts Docetaxel-Induced Cell Cycle Activation, Resulting in Enhanced Efficacy of the Combination 2024 Triple Meeting Update Differential expression analysis was performed comparing the groups: (A) PRT3789 vs Vehicle, (B) docetaxel vs Vehicle, (C) PRT3789+ docetaxel vs Vehicle. Differential expression analysis was performed with the DESeq2 R package6 and Log2 fold change was calculated for the above comparisons. Volcano plots showing the log2 fold change of each gene on the x-axis and the -log10(adjusted p-value) on the y-axis. The sign(log2 fold change) * -log10(p-value) from the above differential expression comparisons was used to rank genes. Hallmarks gene set collection from the Molecular Signatures Database (MSigDB)7,8 was curated using the msigdbr R package9. Analysis performed using Pluto (https://pluto.bio). Hulse M., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
PRT3789 and Docetaxel Combination Induces a Dual G1 and G2/M Arrest and Enhances Apoptosis in SMARCA4-deleted Cells Cell cycle analysis was performed using the Invitrogen™ Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit on the SMARCA4-del NSCLC cell line NCI-H838 (A) and the SMARCA4-WT NSCLC cell line Calu-6 (B) following 48 hr PRT3789 and/or docetaxel for treatment. The isogenic SMARCA4 KO (C) and SMARCA4 WT (D) HT1080 cell lines were dosed with PRT3789 and/or docetaxel for 48 hrs. The Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit was used to determine the apoptotic cell population. Early apoptotic cells were defined as SYTOX-/annexin V+. Late apoptotic cells were defined as SYTOX+/annexin V+. 2024 Triple Meeting Update Hulse M., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
PRT7732 Also Shows High Potential for Synergy With Other Common Anti-Cancer Agents Oral daily administration of PRT7732 in combination with nab-paclitaxel induces tumor regressions in murine tumor xenograft models Shvartsbart, K. Ito et al., AACR Poster, April 2024. (http://www.preludetx.com/science/publications)
SMARCA2 Degraders May Also Help to Potentiate PD1/PDL1 Immunotherapy Confidential Add figure Increased antigen processing “Turning Cold Tumors Hot?” In SMARCA4-deficient cancer cell lines, SMARCA2 degradation… + SMARCA2 Degrader Upregulates antigen processing and presentation machinery Increases cytokine production Promotes T-cell activity and accelerates tumor cell killing Induces presentation of unique MHC-I peptide Increased IFN-γ production Increased T cell activity Anti-PD1 checkpoint blockade
Preclinical Data for PRT3789 Support Rationale for Anti-PD1 Combination Fluorescently labeled tumor cells + PRT3789 + T-cells + T-cells + PRT3789 SMARCA2 Degrader + Anti-PD1 Demonstrates Tumor Regression In Vivo PRT3789 Increases IFN-g Levels in Combination with anti-PD1 In Vitro PRT3789 Upregulates Genes for Antigen Processing and Presentation PRT3789 Promotes T-cell mediated Tumor Cell Killing In Vitro TAP1/2, HLA-A/C/G/F, B2M Anti-PD1 ENA 2023 Poster (http://www.preludetx.com/science/publications); Data on file
Prelude Has Initiated a Phase 2 Combination Study of PRT3789 + Pembrolizumab PRT3789 upregulates genes encoding antigen processing and presentation machinery Trial will explore safety and anti-tumor activity of the combination Preclinical evidence provides rationale for enhanced efficacy with PRT3789 and anti-PD1 therapy combination KEYTRUDA® is a registered trademark of Merck Sharp & Dohme LLC, a subsidiary of Merck & Co., Inc., Rahway, NJ, USA.
Majority of Advanced NSCLC Patients Are Treated with Chemoimmunotherapy Advanced NSCLC Diagnosis (Stage IIIB or IV) Actionable Mutation Identified No Actionable Mutation Identified KRAS, MET, ERBB2, BRAF, ROS, RET, Others EGFR ALK ICI +/- Chemo Chemoimmunotherapy PDL1 > 50% PDL1 < 50% Note: Simplified schematic based on current ESMO and NCCN Clinical Practice Guidelines and current clinical experience; could include combination treatments with bevacizumab, pemetrexed, nab-paclitaxel and others
SMARCA2 Degraders Have Potential to Expand Precision Medicine Access for NSCLC Patients Percent of Total Advanced NSCLC Patients Treated (FUTURE STATE) MET, ERBB2, BRAF, ROS, RET, Others EGFR ALK KRAS SMARCA Chemo +/- Immunotherapy Precision Medicines ILLUSTRATIVE Potentially more patients than ALK, MET, BRAF, ROS and RET combined 1 1 Based on mutational prevalence; Source for current relative patient share: Datamonitor 2023 Lung Cancer Report Reinforces need for comprehensive genomic profiling More patients tested = More patients eligible SMARCA4 mutations already included on most commonly used commercial NGS testing panels
Highly Selective SMARCA2 Degrader Program Discovery Effort & Oral Degrader Program (PRT7732) Preclinical Rationale for Combinations (2024 EORTC-NCI-AACR Symposium Update) Current Treatment Paradigm & Testing Landscape Precision ADCs First Preclinical Proof-of-Concept Data Presented at 2024 EORTC-NCI-AACR Symposium Overview of Prelude’s Precision ADC Program and Next Steps CDK9 Program for Hematologic Malignancies (PRT2527) Background, Unmet Need and Scientific Rationale Early Clinical Safety and PK/PD Data from Phase I Study in Solid Tumors Interim Phase I Update Planned for Major Medical Meeting in Q4 2024 APPENDIX BOLD = New data included in Appendix with this update
Approved ADCs possess payloads with similar mechanisms of action to conventional chemotherapy such as Monomethyl Auristatin E (MMAE) Novel payloads may allow targeting of previously intractable biological pathways (e.g., SMARCA2/4) Novel payloads may open the ADC modality to other cancers that do not currently benefit from targeted therapies Need for Payload Diversification is an Emerging Theme for Next Generation ADCs in the Clinic
Prelude’s Precision ADCs are Designed to Improve the Therapeutic Index Over Traditional ADCs ADC Precision ADC Potency Antibody Selectivity Payload Selectivity PD Marker for Payload Therapeutic Index X Payload Selectivity Highly potent and cell line selective targeted protein degrader Antibody Selectivity Highly selectively antibody that targets cancers that are sensitive to our payload Precision ADC Potential for enhanced potency and selectivity of antibody and payload to improve both efficacy and therapeutic index X X X =
Together, Prelude and AbCellera are Creating Novel, First-in-Class Precision ADCs Multi-year global collaboration to jointly discover, develop and commercialize novel Precision ADCs for up to five programs AbCellera will lead manufacturing activities Prelude will lead clinical development and global commercialization (AbCellera co-promote option) Expertise in chemistry and biology of targeted protein degradation and clinical development capabilities Expertise in antibody discovery, engineering and manufacturing capabilities +
Framework for Precision ADC Differentiation Antibody Differentiation Off-the-shelf / approved mABs Novel, differentiated, highly engineered mABs Payload-Linker Differentiation Non-selective cytotoxin (e.g., DM1, MMAE) Traditional ADCs Precision ADCs Traditional ADCs deliver highly potent and broadly cytotoxic payloads to cells expressing selected cell surface antigens Molecularly targeted degrader “Targeted Times Two” or “Precision2” Key attributes to optimize : Payload selectivity to cancer types highly sensitive to mechanism of action (MOA) Payload potency, selectivity, and half-life to further limit off-target toxicities Linker stability / cleavability Additional patient selection factors based on payload characteristics Key attributes to optimize: Antigen selectivity and binding characteristics Internalization DAR (Drug-Antibody Ratio)
SMARCA2/4 Degrader Payload (PRP0004) Induces Apoptosis and Regulates the Expression of Key Oncoprotein Drivers in Prostate Cancer Cells Triple Meeting Update Prostate cancer was amongst the most sensitive cell lines to SMARCA2/4 degradation rationalizing the use of PSMA-targeting antibodies for further proof-of-concept studies SMARCA2/4 degradation downregulates the expression of several key oncoprotein drivers in prostate cancer cell lines Selective induction of apoptosis in prostate cancer cell lines with a novel payload could lead to an improved therapeutic index Carter J., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
SMARCA2/4 Degrader Payload (PRP0004) Administered On Its Own Induces Tumor Regressions in Prostate Cancer Models, But With a Narrow Therapeutic Index Triple Meeting Update On its own, payload PRP0004 demonstrated dose-dependent tumor growth inhibition in human prostate cancer CDX models However, as anticipated, at higher doses, PRP0004 induced tumor regressions but was limited by a narrow therapeutic index Carter J., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
Anti-PSMA SMARCA2/4 DACs Demonstrate Robust and Significant Antigen-Selective Tumor Growth Inhibition Anti-PSMA SMARCA2/4 DACs were well tolerated and demonstrated robust target engagement and antigen-dependent efficacy in xenograft models These data highlight the potential of utilizing a SMARCA2/4 degrader payload to achieve maximal target degradation in tumors while sparing healthy tissues Precision ADCs have the potential to expand the therapeutic reach of SMARCA2/4 degraders to patients without SMARCA4 mutations 2024 Triple Meeting Update Carter J., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
Degrader Antibody Conjugates (DACs) represent a new frontier in advancing the scientific and clinical potential of antibody drug conjugates (ADCs) Prelude is developing DACs with potent SMARCA2/4 dual degraders as payloads on tumor specific antibodies to achieve maximal target degradation in tumors and spare healthy tissues SMARCA2 and SMARCA4 are the core catalytic subunits of SWI/SNF complexes and play a key role in controlling chromatin remodeling and gene expression Targeting SWI/SNF complexes with targeted protein degraders demonstrates robust anti-tumor activity Because either SMARCA2 or SMARCA4 is necessary for normal cellular functions, maximal suppression of both SMARCA2/4 proteins simultaneously is unlikely to be tolerated Prelude’s SMARCA2/4 dual degraders have shown picomolar potency with potential for increased efficacy, selectivity and a broader therapeutic index leading to a differentiated safety profile Preclinical proof-of-concept has now been presented with novel, highly potent SMARCA2/4 dual degraders conjugated as a “Precision Payloads” to multiple antibodies (PSMA, CEACAM5, TROP-2, C-MET, CD33) DACs expand the reach of SMARCA degrader technology to cancers without SMARCA4 mutations Work is underway to advance first-in-class DAC development candidates from the program and expand our portfolio of novel degrader payloads Overview of Prelude’s Precision ADC Program and Next Steps Carter J., et al., 2024 EORTC-NCI-AACR Symposium Poster (http://www.preludetx.com/science/publications)
Highly Selective SMARCA2 Degrader Program Discovery Effort & Oral Degrader Program (PRT7732) Preclinical Rationale for Combinations (2024 EORTC-NCI-AACR Symposium Update) Current Treatment Paradigm & Testing Landscape Precision ADCs First Preclinical Proof-of-Concept Data Presented at 2024 EORTC-NCI-AACR Symposium Overview of Prelude’s Precision ADC Program and Next Steps CDK9 Program for Hematologic Malignancies (PRT2527) Background, Unmet Need and Scientific Rationale Early Clinical Safety and PK/PD Data from Phase I Study in Solid Tumors Interim Phase I Update Planned for Major Medical Meeting in Q4 2024 APPENDIX BOLD = New data included in Appendix with this update
Source: 1) Maiti A et al. Haematologica 2021. https://doi.org/10.3324/haematol.2020.252569 2) Lew TE et al. Blood Advances 2021. https://doi.org/10.1182/bloodadvances.2021005083 Patients with Hematologic Malignancies Refractory to Current Treatments Experience Poor Outcomes After SoC (venetoclax + HMA), AML patients ineligible for intensive therapy have very poor outcomes (mOS of 2.4 months) Double class (BTKi and BCL2i) resistant CLL is another population with high unmet need (mOS of 3-5 months) Median OS 3.6 months (1) AML (2) CLL
CDK9 Inhibition Targets Two Major Validated Pathways (MYC and MCL-1) CDK9 is the primary transcriptional regulator of a major oncogene MYC and an apoptosis inducer MCL-1 Dysregulated pathways involving MYC and MCL-1 drive pathogenesis and resistance in hematologic cancers including lymphoid and myeloid cancers Prior CDK9i therapies have shown significant GI toxicity, likely driven by poor selectivity across the kinome
PRT2527 is a Potent, Highly Selective CDK9 Inhibitor That Depletes MCL-1 and MYC Highly Isoform Selective CDK9 Inhibitor Compound PRT2527 Biochemical* IC50 (nM) CDK9 0.95 Proliferation* IC50 (nM) 18 Plasma* IC50 (nM) 196 Fold Selectivity CDK9 vs Other Isoforms CDK1 73x CDK2 340x CDK3 35x CDK4 250x CDK5 >1000x CDK6 >1000x CDK7 >1000x >100x 10 -100x PRT2527 177 Assays tested 3 Interactions Mapped S-Score(35) = 0.02 PRT2527 Treatment Depletes MCL-1 and MYC Proteins Highly Selective in Kinome pSer2RNAP2 MCL-1 C-MYC C-Cas3 Actin MV4-11 cell line *Internal data; biochemical assay at 1 mM ATP, H929 CTG proliferation assay ASH 2022 Presentation (https://www.preludetx.com/science/publications)
PRT2527 is Highly Efficacious In Vivo in Models of Hematologic Malignancies Monotherapy Karpas-422 (Double Hit DLBCL) MV4-11 (AML) Combination TMD-8 (ABC DLBCL) OCI-AML3 (VenR AML) ASH 2022 Presentation (https://www.preludetx.com/science/publications); Data on file
Favorable tolerability with manageable neutropenia and absence of significant gastrointestinal events or hepatotoxicity Dose-dependent downregulation of CDK9 transcriptional targets – MYC and MCL-1 mRNA expression in PBMCs isolated from treated patients 12 mg/m2 QW dosing and higher showed optimal target inhibition Overall safety profile observed in this study supported further development of PRT2527 in hematologic malignancies (NCT05665530) Initial Phase 1 Study of PRT2527 in Solid Tumors Evaluated Both Safety and PK/PD Properties Source: Patel, MR et al., AACR-NCI-EORTC 2023, Poster C164 (http://www.preludetx.com/science/publications) PRT2527-Associated Inhibition of CDK9 Transcriptional Targets MYC (A), MCL1 (B) in PBMCs Note: The dotted line represents pre-dose baseline levels. ClinicalTrials.gov Identifier: NCT05159518
Thank You Contact Us: Robert Doody SVP, Investor Relations rdoody@preludetx.com
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na |
| Period Type: |
duration |
|
| X |
- DefinitionThe type of document being provided (such as 10-K, 10-Q, 485BPOS, etc). The document type is limited to the same value as the supporting SEC submission type, or the word 'Other'.
| Name: |
dei_DocumentType |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:submissionTypeItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionAddress Line 1 such as Attn, Building Name, Street Name
| Name: |
dei_EntityAddressAddressLine1 |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:normalizedStringItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- Definition
+ References
+ Details
| Name: |
dei_EntityAddressCityOrTown |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:normalizedStringItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionCode for the postal or zip code
| Name: |
dei_EntityAddressPostalZipCode |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:normalizedStringItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionName of the state or province.
| Name: |
dei_EntityAddressStateOrProvince |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:stateOrProvinceItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionA unique 10-digit SEC-issued value to identify entities that have filed disclosures with the SEC. It is commonly abbreviated as CIK. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection b-2
| Name: |
dei_EntityCentralIndexKey |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:centralIndexKeyItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionIndicate if registrant meets the emerging growth company criteria. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection b-2
| Name: |
dei_EntityEmergingGrowthCompany |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:booleanItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionCommission file number. The field allows up to 17 characters. The prefix may contain 1-3 digits, the sequence number may contain 1-8 digits, the optional suffix may contain 1-4 characters, and the fields are separated with a hyphen.
| Name: |
dei_EntityFileNumber |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:fileNumberItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionTwo-character EDGAR code representing the state or country of incorporation.
| Name: |
dei_EntityIncorporationStateCountryCode |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:edgarStateCountryItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionThe exact name of the entity filing the report as specified in its charter, which is required by forms filed with the SEC. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection b-2
| Name: |
dei_EntityRegistrantName |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:normalizedStringItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionThe Tax Identification Number (TIN), also known as an Employer Identification Number (EIN), is a unique 9-digit value assigned by the IRS. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection b-2
| Name: |
dei_EntityTaxIdentificationNumber |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:employerIdItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionLocal phone number for entity.
| Name: |
dei_LocalPhoneNumber |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:normalizedStringItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionBoolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 13e
-Subsection 4c
| Name: |
dei_PreCommencementIssuerTenderOffer |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:booleanItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionBoolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 14d
-Subsection 2b
| Name: |
dei_PreCommencementTenderOffer |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:booleanItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionTitle of a 12(b) registered security. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection b
| Name: |
dei_Security12bTitle |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:securityTitleItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionName of the Exchange on which a security is registered. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 12
-Subsection d1-1
| Name: |
dei_SecurityExchangeName |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:edgarExchangeCodeItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionBoolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as soliciting material pursuant to Rule 14a-12 under the Exchange Act. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Exchange Act
-Number 240
-Section 14a
-Subsection 12
| Name: |
dei_SolicitingMaterial |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:booleanItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionTrading symbol of an instrument as listed on an exchange.
| Name: |
dei_TradingSymbol |
| Namespace Prefix: |
dei_ |
| Data Type: |
dei:tradingSymbolItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
| X |
- DefinitionBoolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as written communications pursuant to Rule 425 under the Securities Act. Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Securities Act
-Number 230
-Section 425
| Name: |
dei_WrittenCommunications |
| Namespace Prefix: |
dei_ |
| Data Type: |
xbrli:booleanItemType |
| Balance Type: |
na |
| Period Type: |
duration |
|
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