FORM 4
[ ] Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).         
 UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP OF SECURITIES
                                                                                  
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Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940
                      

1. Name and Address of Reporting Person *

DEUTSCHE BANK AG		 2. Issuer Name and Ticker or Trading Symbol

NUVEEN MUNICIPAL HIGH INCOME OPPORTUNITY FUND [ NMZ ] 		5. Relationship of Reporting Person(s) to Issuer (Check all applicable)

_____ Director                    __X__ 10% Owner
_____ Officer (give title below)    _____ Other (specify below)
(Last)          (First)          (Middle)

TAUNUSANLAGE 12 D-60325		 3. Date of Earliest Transaction (MM/DD/YYYY)

6/9/2022
(Street)

FRANKFURT AM MAIN, 2M 00000
(City)        (State)        (Zip)
4. If Amendment, Date Original Filed (MM/DD/YYYY)

 
 6. Individual or Joint/Group Filing (Check Applicable Line)

___ Form filed by One Reporting Person
_ X _ Form filed by More than One Reporting Person

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned
1.Title of Security
(Instr. 3) 		2. Trans. Date 2A. Deemed Execution Date, if any 3. Trans. Code
(Instr. 8) 		4. Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5) 		5. Amount of Securities Beneficially Owned Following Reported Transaction(s)
(Instr. 3 and 4) 		6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4)
Code V Amount (A) or (D) Price
Adjustable Rate MuniFund Term Preferred Shares 6/9/2022  J(1)(2)  1000 A(1) (1)2700 I By Subsidiary (2)(3)

Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivate Security
(Instr. 3)		2. Conversion or Exercise Price of Derivative Security3. Trans. Date3A. Deemed Execution Date, if any4. Trans. Code
(Instr. 8)		5. Number of Derivative Securities Acquired (A) or Disposed of (D)
(Instr. 3, 4 and 5)		6. Date Exercisable and Expiration Date7. Title and Amount of Securities Underlying Derivative Security
(Instr. 3 and 4)		8. Price of Derivative Security
(Instr. 5)		9. Number of derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4)10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4)11. Nature of Indirect Beneficial Ownership (Instr. 4)
CodeV(A)(D)Date ExercisableExpiration DateTitleAmount or Number of Shares
Explanation of Responses:
(1) The 1,000 adjustable rate munifund term preferred shares reported as acquired in Table I represent adjustable rate munifund term preferred shares, series 2032 (the "AMTP Shares") beneficially owned by DB Municipal Holdings LLC ("DBMN") that were acquired from the Issuer at a price of $100,000 per share. Prior to this filing, DBMN held 1,700 adjustable rate munifund term preferred shares, series 2031 issued by the Issuer. DBMN is a wholly owned subsidiary of Deutsche Bank AG.
(2) This statement is jointly filed by Deutsche Bank AG and DBMN. Deutsche Bank AG holds an indirect interest in the securities listed in Table I by virtue of its indirect ownership of DBMN.
(3) Each reporting person declares that neither the filing of this statement nor anything herein shall be construed as an admission that such person is, for the purposes of Section 13(d) of the US Securities Exchange Act of 1934 or any other purpose, (i) acting (or has agreed or is agreeing to act together with any other person) as a partnership, limited partnership, syndicate or other group for the purpose of acquiring, holding or disposing of securities of the Issuer or otherwise with respect to the Issuer or any securities of the Issuer or (ii) a member of any group with respect to the Issuer or any securities of the Issuer.

Remarks:
Exhibits Index
Exhibit 99.1 - Joint Filing Agreement
Exhibit 99.2 - Joint Filer Information

Reporting Owners
Reporting Owner Name / Address
Relationships
Director10% OwnerOfficerOther
DEUTSCHE BANK AG
TAUNUSANLAGE 12 D-60325
FRANKFURT AM MAIN, 2M 00000
X

DB Municipal Holdings LLC
1 COLUMBUS CIRCLE
NEW YORK, NY 10019
X
Signatures
DEUTSCHE BANK AG, by: /s/ David Ramos, /s/ Michael Caro6/13/2022
**Signature of Reporting PersonDate

DB MUNICIPAL HOLDINGS LLC, by: /s/ Svetlana Segal, /s/ John Werba6/13/2022
**Signature of Reporting PersonDate
Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.
*If the form is filed by more than one reporting person, see Instruction 4(b)(v).
**Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
Note:File three copies of this Form, one of which must be manually signed. If space is insufficient, see Instruction 6 for procedure.
Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB control number.
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